Coding
LEV
Part:BBa_K807002:Design
Designed by: Ian Roney Group: iGEM12_uOttawa_CA (2012-09-28)
LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 250
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 112
Illegal BglII site found at 675 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 575
Illegal BsaI.rc site found at 47
Design Notes
LexA DBD was yeast optimized and synthesized by IDT. We had to ensure that LexA DBD and hER did not have stop codons in order for the fusion protein to be built.
Source
LexA DBD - E.coli hER - Human VP16 - Herpes virus